Evaluation of a multi‐agent chemotherapy protocol combining lomustine,procarbazine and prednisolone (LPP) for the treatment of relapsed canine non‐Hodgkin high‐grade lymphomas          下载免费PDF全文

J.‐B. Tanis  S. L. Mason  T. W. Maddox  L. Blackwood  D. R. Killick  I. Amores‐Fuster  A. Harper  R. Finotello 《Veterinary and comparative oncology》2018,16(3):361-369

The standard of care treatment for canine lymphoma is multi‐agent chemotherapy containing prednisolone, cyclophosphamide, vincristine and an anthracycline such as doxorubicin (CHOP) or epirubicin (CEOP). Lomustine, vincristine, procarbazine, and prednisone (LOPP) has been evaluated as a rescue, with encouraging results; however, resistance to vincristine is likely in patients relapsing on CHOP/CEOP, and this agent may enhance LOPP toxicity without improving efficacy. The aim of this study was to evaluate responses to a modified‐LOPP protocol that does not include vincristine (LPP) and is administered on a 21‐day cycle. Medical records of dogs with high‐grade multicentric lymphoma from 2012 to 2017 were reviewed. Dogs with relapsed lymphoma that received LPP as a rescue protocol were enrolled. Response, time from initiation to discontinuation (TTD) and toxicity of LPP were assessed. Forty‐one dogs were included. Twenty‐five dogs (61%) responded to LPP including 12 complete responses (CR) and 13 partial responses (PR). Responders had a significantly longer TTD (P < .001) compared to non‐responders with 84 days for CR and 58 days for PR. Neutropenia was documented in 20 dogs (57%): 12 grade I to II, 8 grade III to IV. Thrombocytopenia was infrequent (20%): 5 grade I to II, 2 grade III to IV. Twelve dogs developed gastrointestinal toxicity (30%): 10 grade I to II and 2 grade III. Nineteen dogs had elevated ALT (59%): 9 grade I to II, 10 grade III to IV. Treatment was discontinued due to toxicity in 8 dogs (19%). The LPP protocol shows acceptable efficacy and toxicity‐profile and minimizes in‐hospital procedures.  相似文献   

In vitro and in vivo effect of flutamide on steroid hormone secretion in canine and human inflammatory breast cancer cell lines          下载免费PDF全文

S. Caceres  B. Monsalve  L. Peña  P. J. de Andres  A. Alonso‐Diez  M. J. Illera  W. A. Woodward  J. M. Reuben  G. Silvan  J. C. Illera 《Veterinary and comparative oncology》2018,16(1):148-158

The aim was to study the effects of flutamide on cell proliferation, in vivo tumour growth and steroid production in canine and human IBC cell lines. IPC‐366 and SUM149 cell cultures were exposed to flutamide concentrations for 72 hours. Additionally, IPC‐366 and SUM149 xenotransplanted mice were treated subcutaneously with flutamide 3 times a week for 2 weeks. Steroid hormones determination in culture media, serum and tumour homogenates (pregnenolone, progesterone, androstenedione, testosterone, dihydrotestosterone, 17β‐oestradiol and oestrone sulphate) were assayed by EIA. in vitro cell proliferation percentages showed a decrease in all flutamide dosages in IPC‐366 and SUM149. in vivo flutamide reduced tumour size by 55% to 65%, and metastasis rates decreased. In treated groups, androgen levels in culture media, serum and tumour homogenates were increased as oestrogen levels decreased. These results suggest that flutamide treatment inhibits cell proliferation and promotes tumour reduction by increasing androgen levels and also support future therapy approaches.  相似文献   

Effects of esmolol on systemic hemodynamics and heart rate variability measured using the Analgesia/Nociception Index in resuscitated piglets with Pseudomonas aeruginosa septic shock          下载免费PDF全文

Emmanuel Boselli MD  PhD  Matthias Jacquet‐Lagrèze MD  Jean‐Yves Ayoub DVM  Lionel Bouvet MD  PhD  Olivier Dauwalder PharmD  PhD  Christelle Mansour DVM  Thomas Margez Eng  Christian Paquet Eng  Damien Restagno MSc  Bernard Allaouchiche MD  PhD  Jeanne‐Marie Bonnet‐Garin DVM  PhD  Stéphane Junot DVM  PhD 《Journal of Veterinary Emergency and Critical Care》2018,28(5):447-456

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Inflammatory cytokine and C‐reactive protein concentrations in dogs with systemic inflammatory response syndrome          下载免费PDF全文

Kris Gommeren DVM  PhD  DECVIM  Isabelle Desmas DVM  DACVIM  Alexandra Garcia DVM  Natalie Bauer DVM  PhD  DECVCP  Andreas Moritz DVM  PhD  DECVIM  Joachim Roth DVM  PhD  Dominique Peeters DVM  PhD  DECVIM 《Journal of Veterinary Emergency and Critical Care》2018,28(1):9-19

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Prognostic significance of clinical presentation,induction and rescue treatment in 42 cases of canine centroblastic diffuse large B‐cell multicentric lymphoma in the United Kingdom          下载免费PDF全文

O. Davies  B. Szladovits  G. Polton  O. A. Garden  C. Leo  A. Lara‐Garcia 《Veterinary and comparative oncology》2018,16(2):276-287

Canine lymphoma is a heterogeneous group of diseases and many previous studies have evaluated the response of a mixed population of lymphoma cases to one specific treatment protocol. The aim of this retrospective study was to describe the outcome and prognostic factors in 42 cases of multicentric centroblastic diffuse large B‐cell lymphoma treated with either a COP‐type (35%) or CHOP‐type (64%) induction chemotherapy. The objective response rate to induction therapy was 94%; entire dogs had a greater rate of complete vs partial remissions than neutered dogs (P = .017). Median progression‐free survival for the first remission (PFS1) was 182 days; absence of anaemia at diagnosis (P = .002) and pretreatment neutrophil:lymphocyte ratio (NLR) below 9.44 (P = .015) were independently predictive of longer PFS1. Fifty‐eight percent of dogs received rescue protocols with an objective response rate of 81%; 31% of dogs received further rescue protocols (up to a total of 5) and the median number of protocols administered were 2. Median overall survival (OS) was 322 days, the 1‐year survival rate was 38% and the 2‐year survival rate was 9%. Lymphocyte:monocyte ratio above 1.43 (P = .031), NLR below 11.44 (P = .009), the combination of induction and rescue therapy (P = .030) and the total number of doxorubicin doses used (P = .002) were independently predictive of longer OS. Use of a COP‐type protocol induction compared with CHOP did not undermine OS providing doxorubicin was used as rescue therapy.  相似文献   

The JAK2/STAT5 signaling pathway as a potential therapeutic target in canine mastocytoma          下载免费PDF全文

Alexandra Keller  Bettina Wingelhofer  Barbara Peter  Karin Bauer  Daniela Berger  Susanne Gamperl  Martin Reifinger  Sabine Cerny‐Reiterer  Richard Moriggl  Michael Willmann  Peter Valent  Emir Hadzijusufovic 《Veterinary and comparative oncology》2018,16(1):55-68

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Changes in the prevalence,genotypes and antimicrobial resistance phenotypes of non‐typhoidal Salmonella recovered from mail‐order hatchling poultry sold at US feed stores, 2013–2015          下载免费PDF全文

A. Sharma  M. M. Erdman  L. Muñoz‐Vargas  D. F. Mollenkopf  G. G. Habing 《Zoonoses and public health》2018,65(1):e102-e112

Every year, multiple outbreaks of salmonellosis in humans are linked to contact with mail‐order chicks and ducks. The objective of this study was to describe the temporal changes in the prevalence of serovars, genotypes and antimicrobial resistance (AMR) phenotypes of non‐typhoidal Salmonella (NTS) recovered from shipped boxes of mail‐order hatchling poultry in the United States during 2013 to 2015. In each year, a sample of feed stores belonging to a single national chain participated in the study. The store employees submitted swabs or hatchling pads from hatchling boxes and shipment tracking information of the arriving boxes to the investigators. NTS was cultured from the samples and isolates were sent to the National Veterinary Services Laboratories (Ames, IA) for serotyping, pulsed‐field gel electrophoresis (PFGE) and AMR phenotyping. The PFGE patterns of Salmonella serovars isolated from hatchling boxes were compared with those from human outbreaks of salmonellosis linked to live poultry contact. The box‐level prevalence of NTS was significantly higher in 2015 compared to 2014. Also, the population of Salmonella serovars recovered in 2015 was more diverse and substantially different from those recovered in the previous two years. Of PFGE patterns recovered from hatchling boxes, seven distinct patterns in 2015, three in 2014 and four in 2013 were indistinguishable from the PFGE patterns of human outbreaks‐associated strains in the respective years. Importantly, a significant positive correlation was found between the box‐level prevalence of PFGE patterns and the number of human illnesses associated with the same patterns. Also, the proportion of multidrug‐resistant isolates was higher in 2014 and 2015 compared to that in 2013. The results demonstrate that shipments of mail‐order hatchling poultry are frequently contaminated with Salmonella genotypes indistinguishable from human outbreaks‐associated strains each year, and control efforts at hatchery level are likely to have an important public health impact.  相似文献   

Risk factors of Leptospira infection in Mediterranean periurban micromammals          下载免费PDF全文

J. Millán  A. Cevidanes  A. D. Chirife  M. G. Candela  L. León‐Vizcaíno 《Zoonoses and public health》2018,65(1):e79-e85

Urbanization of natural areas can change abiotic factors, providing artificial sources of humidity in summer and decreasing variation of temperatures in winter. Our study aimed at document risk factors of infection in mammal reservoirs of pathogenic Leptospira in the human/wildlife interface of a large metropolitan area. We hypothesize that survival of Leptospira and thus their prevalence in animal reservoirs should be higher in residential areas than in natural habitats, especially after the hot, dry Mediterranean summers. We established the prevalence of Leptospira spp. and identified the serovars in 353 urine samples from micromammals (chiefly the wood mouse Apodemus sylvaticus, n = 266) using direct immunofluorescence and PCR. Animals were captured in spring and autumn, 2011–2012, in two natural parks and two adjacent residential areas in periurban Barcelona (NE Spain). Overall observed prevalence of infection was 11%, ranking between 8% and 13% in the better represented host species. We observed marked differences between seasons; the probability of finding a micromammal infected in spring was three times greater than in autumn (almost four times for wood mouse). Prevalence was not related with type of habitat, micromammal relative abundance or sex of the animal. Three Leptospira species were confirmed: Leptospira interrogans (47% of cases), Leptospira borgpetersenii (41%) and Leptospira kirschneri (12%). The serovars most commonly detected were those typically hosted by rodents, and serovars Ballum and Icterohemorrhagiae were the only ones found in autumn. People living in periurban Barcelona and those visiting the natural areas of the metropolitan area face hazard of infection with rodent‐borne Leptospira, especially during spring.  相似文献   

Systematic Review: Impact of point sources on antibiotic‐resistant bacteria in the natural environment          下载免费PDF全文

I. Bueno  J. Williams‐Nguyen  H. Hwang  J. M. Sargeant  A. J. Nault  R. S. Singer 《Zoonoses and public health》2018,65(1):e162-e184

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Length–mass allometries in amphibians          下载免费PDF全文

Luca SANTINI  Ana BENÍTEZ‐LÓPEZ  Gentile Francesco FICETOLA  Mark A. J. HUIJBREGTS 《Integrative zoology》2018,13(1):36-45

Body mass is rarely recorded in amphibians, and other body measurements (e.g. snout to vent length, SVL) are generally collected instead. However, length measurements, when used as proxies of body mass in comparative analyses, are problematic if different taxa and morphotypes are included. We developed allometric relationships to derive body mass from SVL measurements. We fitted phylogenetic generalized least square models for frogs (Anura) and salamanders (Caudata) and for several families separately. We tested whether allometric relationships differed between species with different habitat preferences and between morphs in salamanders. Models were fitted with SVL–mass measurements for 88 frog and 42 salamander species. We assessed the predictive performance of the models by cross‐validation. Overall, the models showed high explained variance and low forecasting errors. Models differed among semi‐aquatic, terrestrial and arboreal frogs, and between paedomorphic and non‐paedomorphic salamanders. Body mass estimates derived from our models allow for comparability of studies on multiple taxa and can be used for testing theories built upon evolutionary and ecological processes which are directly related to body mass.  相似文献